Binder C 150 User Manual download pdf (Page 60)

C 150 (E2) 12/2012 page 60/90

Examples of general rules to reduce the contamination risks

Reduce hand germ count (wash hands with antimicrobial soap, dry with paper tissues, and rub dry

hands with alcoholic solution).

Wear appropriate clothing (work coat, shoes, face mask)

Keep as few personnel as possible in the cell culture lab.

Examples of sterile working method

Work "clean-to-dirty", i.e., handle confirmed uncontaminated cells first, unknown or untested cells next,

and lastly, if necessary, cells suspected to be contaminated.

Perform daily microscopic observations of cultures and specific tests for the bacteria and fungi as part

of a controlled routine. Test cultures for sterility before starting work.

Keep working surfaces clean. Immediately wipe spilled liquids with alcohol solutions.

No mouth contact on pipettes.

Never work on top of open sterile containers.

14.4 Chamber design and equipment of the CO

incubator C 150

The design concepts behind the CO

incubator C 150 considerably reduce the risk of contamination.

Among them are:

Even surfaces for easy manual cleaning

The inner surfaces are smooth and therefore easy to clean. The inner chamber is deep-drawn from a

single piece of steel, polished (suitable for pharmaceutical work) and has no welds or inaccessible

corners. The hinges and the seal of the inner glass door are glued from the outside, which also aids

cleaning of the inner chamber.

Removable parts for cleaning and autoclaving

The shelves are easily removed without screws. It is possible to autoclave the shelves. But this is

generally not necessary because they can remain inside the incubator during sterilization.

Door gasket

The inner door gasket is removable and autoclavable.

Gas fine filter

The incoming gas used in the operation passes through a fine filter (aseptic filter, filtration efficiency

99.99%, particle size 0.45 m) with a high filtration efficiency, which can also filter the smallest parti-

cles (chap. 16.3).

measuring system in the inner chamber

You can remove the CO

sensor from the inner chamber by hand for disinfection (chap. 15.3).

Condensation prevention

Condensation in the inner chamber represents a particular risk of contamination. The humidifying sys-

tem with water pan with integrated condensation point developed by BINDER is an effective and easy

way to ensure high humidity (95 ± 2% r.H.) inside the incubator without any condensation forming on

the inner surfaces.

Hot air sterilization at 180 °C / 356 °F

The heating system of the C 150 permits hot-air auto-sterilization (chap. 15.4). Thus, a temperature of

180 °C / 356°F is maintained for a 30-minute period on all internal surfaces during its cycle, resulting in

sterilization of the entire inner chamber. Therefore, this procedure meets all international guidelines

regarding hot air sterilization, e.g. AAMI ST63, DIN 58947, European Pharmacopoeia.

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