Binder C 150 User Manual

Issue 12/2012 Art. No. 7001-0172 Operating Manual APT.line™ C 150 (E2) CO2 Incubators with FPI-sensor system and display controller RP1 Mo

C 150 (E2) 12/2012 page 10/90 WARNING Indicates a potentially hazardous situation which, if not avoided, could result in death or serious

C 150 (E2) 12/2012 page 11/90 Prohibition signs Do NOT touch Do NOT spray with water Do NOT climb Information to be observed in ord

C 150 (E2) 12/2012 page 12/90 Figure 3: Position of labels on the CO2 incubator C 150-UL (door hinged right) Figure 4: Posit

C 150 (E2) 12/2012 page 13/90 Figure 6: Type plate C 150 (standard unit) Indications of the type plate Information BINDER Manufacturer:

C 150 (E2) 12/2012 page 14/90 1.5 General safety instructions on installing and operating the CO2 incubator With regard to operating the

C 150 (E2) 12/2012 page 15/90 The CO2 incubators were produced in accordance with VDE regulations and were routinely tested in ac-cordance

C 150 (E2) 12/2012 page 16/90 1.7 Precautions when handling gas cylinders General information for safe handling of gas cylinders: Stor

C 150 (E2) 12/2012 page 17/90 Always use proper protective equipment (clothing, gloves, safety glasses, etc.). Always follow good hygieni

C 150 (E2) 12/2012 page 18/90 2.2 Unit overview Figure 7: CO2 incubator C 150 (door hinged right) (1) Triangle instrument panel with

C 150 (E2) 12/2012 page 19/90 2.3 Connection panel at the rear of the unit (11) (12) (13) (14) (15) Figure 8: Rear connection pa

C 150 (E2) 12/2012 page 2/90 EC – declaration of conformity EG – KONFORMITÄTSERKLÄRUNG EC - DECLARATION OF CONFORMITY CE - DECLARATION

C 150 (E2) 12/2012 page 20/90 2.5 Unit doors The outer door of the C 150 is equipped with a heater on its inner side. The door must be cl

C 150 (E2) 12/2012 page 21/90 3.2 Guidelines for safe lifting and transportation After operation, please observe the guidelines for tempo

C 150 (E2) 12/2012 page 22/90 3.3 Storage Intermediate storage of the unit is possible in a closed and dry room. Observe the guidelines f

C 150 (E2) 12/2012 page 23/90 Avoid direct solar radiation on the unit. Permissible ambient humidity: 70% r.H. max., non-condensing.

C 150 (E2) 12/2012 page 24/90 4.2 CO2 sensor 4.2.1 General notes Connect or remove the CO2 sensor without rotating and only when the inc

C 150 (E2) 12/2012 page 25/90 4.3 Water pan The water pan permits high humidity without condensation on the inner walls of the incubator.

C 150 (E2) 12/2012 page 26/90 The valve of the gas cylinder always must be closed before screwing on or unscrewing the gas hose. WARNIN

C 150 (E2) 12/2012 page 27/90 The following steps are required: Ensuring the correct CO2 output pressure A gas supply pressure above 2.5

C 150 (E2) 12/2012 page 28/90 4.4.2 Connecting the gas hose to the unit rear The gas hose, which will be used to establish the connection

C 150 (E2) 12/2012 page 29/90 4.4.3 Gas cylinder connection kit (option) The gas cylinder connection kit for CO2 (Art. no. 8012-

C 150 (E2) 12/2012 page 3/90 Die oben beschriebenen Produkte sind konform mit folgenden harmonisierten Normen: The products described abov

C 150 (E2) 12/2012 page 30/90 4.5 Electrical connection The CO2 incubator C 150 has a fixed power supply connection cable 1800 mm / 70.

C 150 (E2) 12/2012 page 31/90 5. Start up After connecting the supply lines, turn on the unit by the main power switch (2). After turning

C 150 (E2) 12/2012 page 32/90 Figure 18: Normal Display of controller Upper display: current inner chamber temperature LED “ °C” li

C 150 (E2) 12/2012 page 33/90 Open the CO2 supply's pressure reducer valve and set a CO2 primary pressure of 2.0 bar / 29 psi. Set th

C 150 (E2) 12/2012 page 34/90 7.1 Selecting and setting the operating functions The controller's lower display always shows the oper

C 150 (E2) 12/2012 page 35/90 Operating functions that can be set in operating mode HAND Display Setting range Operating function 0.0 °C

C 150 (E2) 12/2012 page 36/90 7.3 Operating mode USER: Advanced settings In Normal display, press “MODE” and “EXIT” simultaneously for

C 150 (E2) 12/2012 page 37/90 Operating functions in operating mode USER Display Setting range Operating function 0.0 °C to 50.0 °C Temp

C 150 (E2) 12/2012 page 38/90 Display Setting range Operating function 0.0 °C to 60.0 °C Safety controller set point when set point type

C 150 (E2) 12/2012 page 39/90 Display Setting range Operating function Firmware revision of main controller for service / maintenance pu

C 150 (E2) 12/2012 page 4/90 D-78532 Tuttlingen, 21.08.2012 BINDER GmbH P. M. Binder Geschäftsführender Gesellschafter Managing Directo

C 150 (E2) 12/2012 page 40/90 7.4 Operating mode LOCK: Locking/unlocking of the operating functions’ set-tings by operating mode HAND To

C 150 (E2) 12/2012 page 41/90 Enter the password using the “▲” and “▼”buttons. Confirm the entry with “MODE”. The controller returns t

C 150 (E2) 12/2012 page 42/90 Toggle to operating mode USER Operating mode HAND Operating mode USER If operating mode HAND appe

C 150 (E2) 12/2012 page 43/90 8.2 Entering the set points of temperature and CO2 Normal Display Entry of temperature set point

C 150 (E2) 12/2012 page 44/90 Note when setting high gas concentrations Carbon dioxide (CO2) in high concentrations is hazardous to health

C 150 (E2) 12/2012 page 45/90 The settings of the safety controller are inactive during sterilization (chap. 15.4). They become functiona

C 150 (E2) 12/2012 page 46/90 12 x Selecting the set point type Selecting set point type “Limit” Change using the arrow keys Selectin

C 150 (E2) 12/2012 page 47/90 Toggle to operating mode USER Operating mode HAND Operating mode USER If operating mode HAND appe

C 150 (E2) 12/2012 page 48/90 10. Alarm functions 10.1 Alarm functions overview When operational faults occur, the controller triggers v

C 150 (E2) 12/2012 page 49/90 10.3 Zero-voltage relay alarm output Collective alarm output via the zero-voltage relay alarm contact The C

C 150 (E2) 12/2012 page 5/90 Product registration

C 150 (E2) 12/2012 page 50/90 10.4 Safety controller temperature alarm The set temperature value (set point type “Limit” or “Offset”) was

C 150 (E2) 12/2012 page 51/90 Temperature too low (under temperature alarm): Check that the outer door is closed properly. Check the d

C 150 (E2) 12/2012 page 52/90 10.7 CO2 tolerance range alarm (CO2 over/under concentration) The main controller CO2 alarm. The CO2 concen

C 150 (E2) 12/2012 page 53/90 Actions: Check that you have set the pressure on the pressure reducer at 2.0 bar / 29 psi above the ambien

C 150 (E2) 12/2012 page 54/90 10.10 Failure of CO2 sensor A sensor fault alarm display takes priority over all other operational disp

C 150 (E2) 12/2012 page 55/90 12. Reference measurements You can take reference measurements of the temperature and CO2 via the silicone

C 150 (E2) 12/2012 page 56/90 Example: If a pH of 7.2 is measured in a medium buffered with 2.20 g NaHC03 per liter, there must be 8 vol.-

C 150 (E2) 12/2012 page 57/90 12.1.3 Measuring CO2 directly with an electronic infrared measuring device The easiest way of measuring the

C 150 (E2) 12/2012 page 58/90 WARNING High concentration of CO2 (> 4 Vol.-%). Risk of death by suffocation. Danger of poisoning. 

C 150 (E2) 12/2012 page 59/90 14. Avoiding microbial contamination The main types of microbial contaminants in cell and tissue culture ar

C 150 (E2) 12/2012 page 6/90 Contents EC – declaration of conformity ...

C 150 (E2) 12/2012 page 60/90 Examples of general rules to reduce the contamination risks Reduce hand germ count (wash hands with antimi

C 150 (E2) 12/2012 page 61/90 14.5 Handling the CO2 incubator C 150 Any manipulation of the CO2 incubator involves some contamination ris

C 150 (E2) 12/2012 page 62/90 15. Cleaning, decontamination / disinfection, and sterilization DANGER Electrical hazard. Danger of de

C 150 (E2) 12/2012 page 63/90 With every cleaning method, always use adequate personal safety controls. Following cleaning, leave the uni

C 150 (E2) 12/2012 page 64/90 In case of eye contact, the disinfectant spray may cause eye damage due to chemical burns. Follow the opera

C 150 (E2) 12/2012 page 65/90 Recommended procedure: Turn off the unit Pull out the sensor Spray the sensor head with alcohol or w

C 150 (E2) 12/2012 page 66/90 The precise duration of the entire sterilization cycle depends on the ambient temperature at the installa-ti

C 150 (E2) 12/2012 page 67/90 Figure 33: Initial sterilization display No sterilization can be started if the CO2 sensor is still

C 150 (E2) 12/2012 page 68/90 CAUTION The glass door and inner chamber become hot during sterilization. Danger of burning. Do NOT touc

C 150 (E2) 12/2012 page 69/90 CAUTION Glass door and inner chamber become hot during sterilization. Danger of burning. Do NOT touch th

C 150 (E2) 12/2012 page 7/90 8. CONTROLLER RP1 SETTINGS ... 41 8.

C 150 (E2) 12/2012 page 70/90 After a manual abortion, the C 150 reverts to its standard operational status. The indication “ - - - “ in

C 150 (E2) 12/2012 page 71/90 16. Maintenance, and service 16.1 Maintenance intervals, service DANGER Electrical hazard. Danger of de

C 150 (E2) 12/2012 page 72/90 16.4 Sending the unit back to BINDER GmbH If you return a BINDER product to us for repair or any other reas

C 150 (E2) 12/2012 page 73/90 17.1.2 Packing inside the unit, equipment Packing element Material Disposal Door protection PE foam Plastic

C 150 (E2) 12/2012 page 74/90 CAUTION Violation against existing law. Do NOT dispose of BINDER devices at public collecting points. 

C 150 (E2) 12/2012 page 75/90 CAUTION Violation against existing law. Do NOT dispose of BINDER devices at public collecting points. 

C 150 (E2) 12/2012 page 76/90 18. Troubleshooting Fault description Possible cause Required measures General Unit without function. No p

C 150 (E2) 12/2012 page 77/90 Fault description Possible cause Required measures Heating (continued) Temperature inside too low. Message

C 150 (E2) 12/2012 page 78/90 Fault description Possible cause Required measures Heating (continued) Unusually high gas consump-tion. Doo

C 150 (E2) 12/2012 page 79/90 19. Technical description 19.1 Factory calibration and adjustment This unit was calibrated and adjusted

C 150 (E2) 12/2012 page 8/90 16. MAINTENANCE, AND SERVICE ... 71 1

C 150 (E2) 12/2012 page 80/90 19.4 C 150 technical data Exterior dimensions Width mm / inch 680 / 26.77 Height (incl. feet) mm / inch 819

C 150 (E2) 12/2012 page 81/90 C 150-UL electrical connection data (for the USA and Canada) Electrical Data IP system of protection acc. to

C 150 (E2) 12/2012 page 82/90 19.7 Equipment and options C 150 To operate the CO2 incubator, use only original BINDER accessories or acc

C 150 (E2) 12/2012 page 83/90 19.8 Accessories and spare parts BINDER GmbH is responsible for the safety features of the unit only, prov

C 150 (E2) 12/2012 page 84/90 19.9 Dimensions C 150

C 150 (E2) 12/2012 page 85/90 20. Contamination clearance certificate Unbedenklichkeitsbescheinigung 20.1 For units located outside Nort

C 150 (E2) 12/2012 page 86/90 3.3 Measures to be taken in case of skin contact or release into the atmosphere / Maßnahmen bei Personenkon

C 150 (E2) 12/2012 page 87/90 We hereby declare that the following measures have been taken / Wir erklären, dass folgende Ma

C 150 (E2) 12/2012 page 88/90 20.2 For units in North America and Central America Product Return Authorization Request Please complete

C 150 (E2) 12/2012 page 89/90 Customer (End User) Decontamination Declaration Health and Hazard Safety declaration To protect the health

C 150 (E2) 12/2012 page 9/90 Dear Customer, For the correct operation of the CO2 incubator C 150, it is important that you read this opera

C 150 (E2) 12/2012 page 90/90 4. Declaration of Decontamination For toxic, radioactive, biologically and chemically harmful or hazardous