Binder C 150 User Manual download pdf (Page 17)

C 150 (E2) 12/2012 page 17/90

Always use proper protective equipment (clothing, gloves, safety glasses, etc.).

Always follow good hygienic practices according to GLP/SOP protocols.

Each individual operating the C 150 incubator is responsible for his or her own safety

2. Unit description

The CO

incubators C 150 are equipped with a microprocessor controller for temperature and CO

levels

and a digital display accurate to one-tenth of a degree resp. 0.1 vol.-%

The inner chamber, the pre-heating chamber and the inside of the doors are all made of stainless steel

(material no. 1.4301 (V2A) in Germany). The inner surfaces are smooth and therefore easy to clean. The

inner chamber is deep-drawn from one piece, polished (suitable for pharmaceutical applications) and has

no welds or inaccessible corners. The hinges and the seal of the inner glass door are glued from the out-

side to aid cleaning of the inner chamber. When operating the chamber at high temperatures (steriliza-

tion), the impact of the oxygen in the air may cause discoloration of the metallic surfaces (yellowish-brown

or blue) by natural oxidation processes. These colorations are harmless and will in no way impair the

function or quality of the unit.

The perforated shelves are made of stainless steel. You can insert a maximum of six shelves.

The housing is RAL 7035 powder-coated. All corners and edges are also completely coated.

The heating system of the CO

incubator permits hot-air auto-sterilization at 190 °C / 374°F. Thus, a tem-

perature of at least 180 °C / 356°F is maintained on all internal surfaces, resulting in sterilization of the

entire inner chamber.

Thanks to the standard safety device (class 3.1 according to DIN 12880), the set temperature is main-

tained in case of failure.

The gas enters the chamber via a fine filter (aseptic filter) with a high filtration efficiency that also filters

the smallest particles.

A highly precise, drift-free CO

infrared measuring system in combination with the permanent mixture of

gas through a special proprietary gas mixing head developed by BINDER allows precise and con-

stant CO

concentrations for long periods. This creates optimum growth conditions for cultures. The CO

sensor can be removed from the inner chamber by hand and cleaned with suitable detergents if needed.

The CO

incubator can be operated in a temperature range from 7 degrees above ambient temperature

up to +50 °C / 122°F and a CO

range of 0 vol.-% up to 20 vol.-%.

2.1 The CO

measuring principle

Fast reaction times, as well as the highest accuracy and selectivity, characterize the CO

measuring pro-

cedure of the C 150 incubator series. The accuracy of the CO

measuring system is based on a single-

beam infrared measuring cell, which measures in differential mode using the permanently alternating

transmission feature of its semi-conductor filter.

Due to this highly developed single-beam principle with Fabry-Perot interferometer (FPI), disturbance

variables and aging phenomena in the measuring system are almost completely eliminated, so that this

measuring system, in contrast to other measuring procedures, remains practically drift-free between cali-

brations and is absolutely selective for CO

The CO

measuring cell contains a measuring section inside, in which the absorption of infrared light

depends on the number of CO

molecules in the beam path. This number of CO

molecules changes with

the ambient pressure in relation to a constant volume. The distances between the molecules are conse-

quently pressure-dependent. The collision frequency of the IR-beam with CO

molecules increases there-

fore by increasing pressure. For this reason, the ambient pressure must be compensated in order to cor-

rect the display reading of the CO

concentration in vol.-%. This is achieved by entering the altitude of the

site above the sea (chap. 8.1).

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Binder C 150 User Manual Page 17

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