Binder C 150 User Manual download pdf (Page 16)

C 150 (E2) 12/2012 page 16/90

1.7 Precautions when handling gas cylinders

General information for safe handling of gas cylinders:

Store and use gas cylinders only in well-ventilated locations.

Open the gas cylinder valve slowly to avoid pressure surges

Secure gas cylinders during storage and use against falling (chaining).

Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them.

Always close the valve even with apparently empty cylinders; screw on the cap when not in

use. Return gas cylinders with the valve closed.

Do not open gas cylinders by force. Mark them when damaged

Protect gas cylinders against fire, e.g. do not store together with flammable liquids

Observe relevant regulations for dealing with gas cylinders.

Secure the gas cylinders against falling and other mechanical damage.

WARNING

Safety valve tearing off.

Sudden release of the stored pressure energy.

Risk of injury.

 Secure gas cylinders against falling (chaining).

 Transport gas cylinders with a cylinder cart.

The valve of the gas cylinder always must be closed before screwing on or unscrewing the gas hose.

WARNING

Opening the cylinder valve when the cylinder is not connected.

Sudden release of the stored pressure energy.

Risk of injury.

 Close the gas cylinder valve before connecting or removing the gas hose.

After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or

diluted soap solution).

1.8 Intended use

Series C 150 incubators are suitable for the cultivation of mammal cells under typical conditions of ap-

prox. 37 °C / 98.6 °F. The incubator permits setting defined pH conditions by common NaHCO

buffer

systems of commercial cell media by keeping an exact CO

atmosphere inside. C 150 incubators guaran-

tee high humidity inside to avoid osmolarity increasing caused by the evaporation of the cell media.

Observing the instructions in this operating manual and conducting regular maintenance work

(chap. 16) is part of the intended use.

Other applications are not approved.

WARNING: If customer should use a BINDER chamber running in non-supervised continu-

ous operation, we strongly recommend in case of inclusion of irrecoverable specimen or

samples to split such specimen or samples and store them in at least two chambers, if this is

feasible.

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Binder C 150 User Manual Page 16